nmda-receptor antagonist cpp Search Results


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Tocris superfusing acsf ttx
(a) Plot of number of mEPSCs versus membrane capacitance. Cells were classified as NG2+ cells, Pre-OLs or OLs based on DsRed fluorescence, membrane capacitance, membrane resistance, and NaV current density. (b) Graph in a colored according to whether NaV current was detected, showing the tight correlation between loss of synapses and loss of NaV current. (c) HS-response of cells indicated by numbers in a and b. (d) Response of a representative NG2+ cell to glutamate uncaging (black dot), recorded at −80 mV and 40 mV (in NBQX/GYKI53655). (e) Response of a representative Pre-OL to glutamate uncaging at −80 mV and 40 mV (in NBQX/GYKI53655). (f) Response of representative OLs to glutamate uncaging recorded at −80 mV showing effects of NBQX/GYKI53655, RS-CPP (upper trace, 0 <t>Mg++ACSF/3</t> μm D-serine), and TBOA (lower trace, normal ACSF); Insets show initial response at faster time scale (bar = 20 ms). (g) Quantification of peak AMPAR current normalized to membrane capacitance for NG2+ cells (n = 8), Pre-OLs (n=8), and OLs (n = 13); red asterisk: NG2+ cell vs. Pre-OL and NG2+ cell vs. OL, P < 0.001; blue asterisk: OL vs. Pre-OL, P < 0.001.
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(a) Plot of number of mEPSCs versus membrane capacitance. Cells were classified as NG2+ cells, Pre-OLs or OLs based on DsRed fluorescence, membrane capacitance, membrane resistance, and NaV current density. (b) Graph in a colored according to whether NaV current was detected, showing the tight correlation between loss of synapses and loss of NaV current. (c) HS-response of cells indicated by numbers in a and b. (d) Response of a representative NG2+ cell to glutamate uncaging (black dot), recorded at −80 mV and 40 mV (in NBQX/GYKI53655). (e) Response of a representative Pre-OL to glutamate uncaging at −80 mV and 40 mV (in NBQX/GYKI53655). (f) Response of representative OLs to glutamate uncaging recorded at −80 mV showing effects of NBQX/GYKI53655, RS-CPP (upper trace, 0 <t>Mg++ACSF/3</t> μm D-serine), and TBOA (lower trace, normal ACSF); Insets show initial response at faster time scale (bar = 20 ms). (g) Quantification of peak AMPAR current normalized to membrane capacitance for NG2+ cells (n = 8), Pre-OLs (n=8), and OLs (n = 13); red asterisk: NG2+ cell vs. Pre-OL and NG2+ cell vs. OL, P < 0.001; blue asterisk: OL vs. Pre-OL, P < 0.001.
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Columbus Instruments animal activity meter opto-varimex-5 auto-track
(a) Plot of number of mEPSCs versus membrane capacitance. Cells were classified as NG2+ cells, Pre-OLs or OLs based on DsRed fluorescence, membrane capacitance, membrane resistance, and NaV current density. (b) Graph in a colored according to whether NaV current was detected, showing the tight correlation between loss of synapses and loss of NaV current. (c) HS-response of cells indicated by numbers in a and b. (d) Response of a representative NG2+ cell to glutamate uncaging (black dot), recorded at −80 mV and 40 mV (in NBQX/GYKI53655). (e) Response of a representative Pre-OL to glutamate uncaging at −80 mV and 40 mV (in NBQX/GYKI53655). (f) Response of representative OLs to glutamate uncaging recorded at −80 mV showing effects of NBQX/GYKI53655, RS-CPP (upper trace, 0 <t>Mg++ACSF/3</t> μm D-serine), and TBOA (lower trace, normal ACSF); Insets show initial response at faster time scale (bar = 20 ms). (g) Quantification of peak AMPAR current normalized to membrane capacitance for NG2+ cells (n = 8), Pre-OLs (n=8), and OLs (n = 13); red asterisk: NG2+ cell vs. Pre-OL and NG2+ cell vs. OL, P < 0.001; blue asterisk: OL vs. Pre-OL, P < 0.001.
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(a) Plot of number of mEPSCs versus membrane capacitance. Cells were classified as NG2+ cells, Pre-OLs or OLs based on DsRed fluorescence, membrane capacitance, membrane resistance, and NaV current density. (b) Graph in a colored according to whether NaV current was detected, showing the tight correlation between loss of synapses and loss of NaV current. (c) HS-response of cells indicated by numbers in a and b. (d) Response of a representative NG2+ cell to glutamate uncaging (black dot), recorded at −80 mV and 40 mV (in NBQX/GYKI53655). (e) Response of a representative Pre-OL to glutamate uncaging at −80 mV and 40 mV (in NBQX/GYKI53655). (f) Response of representative OLs to glutamate uncaging recorded at −80 mV showing effects of NBQX/GYKI53655, RS-CPP (upper trace, 0 <t>Mg++ACSF/3</t> μm D-serine), and TBOA (lower trace, normal ACSF); Insets show initial response at faster time scale (bar = 20 ms). (g) Quantification of peak AMPAR current normalized to membrane capacitance for NG2+ cells (n = 8), Pre-OLs (n=8), and OLs (n = 13); red asterisk: NG2+ cell vs. Pre-OL and NG2+ cell vs. OL, P < 0.001; blue asterisk: OL vs. Pre-OL, P < 0.001.
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(a) Plot of number of mEPSCs versus membrane capacitance. Cells were classified as NG2+ cells, Pre-OLs or OLs based on DsRed fluorescence, membrane capacitance, membrane resistance, and NaV current density. (b) Graph in a colored according to whether NaV current was detected, showing the tight correlation between loss of synapses and loss of NaV current. (c) HS-response of cells indicated by numbers in a and b. (d) Response of a representative NG2+ cell to glutamate uncaging (black dot), recorded at −80 mV and 40 mV (in NBQX/GYKI53655). (e) Response of a representative Pre-OL to glutamate uncaging at −80 mV and 40 mV (in NBQX/GYKI53655). (f) Response of representative OLs to glutamate uncaging recorded at −80 mV showing effects of NBQX/GYKI53655, RS-CPP (upper trace, 0 <t>Mg++ACSF/3</t> μm D-serine), and TBOA (lower trace, normal ACSF); Insets show initial response at faster time scale (bar = 20 ms). (g) Quantification of peak AMPAR current normalized to membrane capacitance for NG2+ cells (n = 8), Pre-OLs (n=8), and OLs (n = 13); red asterisk: NG2+ cell vs. Pre-OL and NG2+ cell vs. OL, P < 0.001; blue asterisk: OL vs. Pre-OL, P < 0.001.
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Image Search Results


(a) Plot of number of mEPSCs versus membrane capacitance. Cells were classified as NG2+ cells, Pre-OLs or OLs based on DsRed fluorescence, membrane capacitance, membrane resistance, and NaV current density. (b) Graph in a colored according to whether NaV current was detected, showing the tight correlation between loss of synapses and loss of NaV current. (c) HS-response of cells indicated by numbers in a and b. (d) Response of a representative NG2+ cell to glutamate uncaging (black dot), recorded at −80 mV and 40 mV (in NBQX/GYKI53655). (e) Response of a representative Pre-OL to glutamate uncaging at −80 mV and 40 mV (in NBQX/GYKI53655). (f) Response of representative OLs to glutamate uncaging recorded at −80 mV showing effects of NBQX/GYKI53655, RS-CPP (upper trace, 0 Mg++ACSF/3 μm D-serine), and TBOA (lower trace, normal ACSF); Insets show initial response at faster time scale (bar = 20 ms). (g) Quantification of peak AMPAR current normalized to membrane capacitance for NG2+ cells (n = 8), Pre-OLs (n=8), and OLs (n = 13); red asterisk: NG2+ cell vs. Pre-OL and NG2+ cell vs. OL, P < 0.001; blue asterisk: OL vs. Pre-OL, P < 0.001.

Journal:

Article Title: Excitability and Synaptic Communication within the Oligodendrocyte Lineage

doi: 10.1523/JNEUROSCI.6000-09.2010

Figure Lengend Snippet: (a) Plot of number of mEPSCs versus membrane capacitance. Cells were classified as NG2+ cells, Pre-OLs or OLs based on DsRed fluorescence, membrane capacitance, membrane resistance, and NaV current density. (b) Graph in a colored according to whether NaV current was detected, showing the tight correlation between loss of synapses and loss of NaV current. (c) HS-response of cells indicated by numbers in a and b. (d) Response of a representative NG2+ cell to glutamate uncaging (black dot), recorded at −80 mV and 40 mV (in NBQX/GYKI53655). (e) Response of a representative Pre-OL to glutamate uncaging at −80 mV and 40 mV (in NBQX/GYKI53655). (f) Response of representative OLs to glutamate uncaging recorded at −80 mV showing effects of NBQX/GYKI53655, RS-CPP (upper trace, 0 Mg++ACSF/3 μm D-serine), and TBOA (lower trace, normal ACSF); Insets show initial response at faster time scale (bar = 20 ms). (g) Quantification of peak AMPAR current normalized to membrane capacitance for NG2+ cells (n = 8), Pre-OLs (n=8), and OLs (n = 13); red asterisk: NG2+ cell vs. Pre-OL and NG2+ cell vs. OL, P < 0.001; blue asterisk: OL vs. Pre-OL, P < 0.001.

Article Snippet: The following agents were applied by addition to the superfusing ACSF: TTX (NaV antagonist; Ascent Scientific, 1 μM; gabazine (SR-95531; GABA receptor antagonist; Tocris, 5μM; RS-CPP (NMDA receptor antagonist; Tocris, 5μM or 20 μM for uncaging); 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline (NBQX; competitive AMPA/kainate receptor antagonist; Tocris, 5 μM or 50 μM for uncaging); GYKI 53655 (AMPA selective, non-competitive antagonist; IVAX 100 μM; TBOA (competitive glutamate transporter blocker; Tocris, 300 μM).

Techniques: Membrane, Fluorescence